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IIS 7.5 Error 401.3

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 I have two servers, Win 2008 R2, that for the most part are configured the same.

On the second server, when users try to access a web site, they get Error 401.3.  I can get rid of that error by giving the  Application Pool "Dev1" access to the file folders that contain the web site.

On the first server, I do not have to do that. Both servers have many web sites, with application pools for each.  They use .Net Framework, Integrated pipeline, and Connect As a specific user.  On the first server, it is a domain account, on the second a local account.

What should I configure different on the second server so that I do not have to specifically grant the application pool rights on the folder?  I am not exactly sure where to look for this, as logging and testing has not given me any clues.


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