I have two servers, Win 2008 R2, that for the most part are configured the same.
On the second server, when users try to access a web site, they get Error 401.3. I can get rid of that error by giving the Application Pool "Dev1" access to the file folders that contain the web site.
On the first server, I do not have to do that. Both servers have many web sites, with application pools for each. They use .Net Framework, Integrated pipeline, and Connect As a specific user. On the first server, it is a domain account, on the second a local account.
What should I configure different on the second server so that I do not have to specifically grant the application pool rights on the folder? I am not exactly sure where to look for this, as logging and testing has not given me any clues.